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Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) <t>TIMP1.</t> Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.
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Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) <t>TIMP1.</t> Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.
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Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques: Western Blot